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KMID : 0364820070430030222
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Korean Journal of Microbiology
2007 Volume.43 No. 3 p.222 ~ p.226
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Isolation and Charcterization of Micrococcus sp. HJ-19 Secreting Extracellular Protease
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Cha In-Tae
Oh Yong-Sik
Roh Dong-Hyun
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Abstract
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Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly
applied to commercial field. In order to screen new source of protease, bacteria secreting extracellular protease were isolated by enrichment culture from deep sea water samples of East Sea, Korea. A bacterium, named as HJ19, showed the best growth and the largest clear zone in plates supplemented skim milk at 30oC. The partial DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology identified that this strain was in genus Micrococcus. The strain HJ19 could not grow at 10oC but it started growth and showed protease activity at 20oC. The optimal growth was at 37oC and the maximal protease activity at 30oC was about 480 unit/ml.
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KEYWORD
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extracellular protease, growth characterization, Micrococcus sp., 16S rRNA gene
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